Densitometric analysis of Western blot (immunoblot) assays for human immunodeficiency virus antibodies and correlation with clinical status
نویسندگان
چکیده
منابع مشابه
Quantification Analysis of Dot Blot Assays for Human Immunodeficiency Virus Type 1 and 2 Antibodies
Objective Dot Blot (DB) assay provides highly specific results, but usually not reliable for quantification of antibody production. The need for a more objective DB assay to provide a better definition of the immune status, against HIV antigens, promoted this study to be done to develop a quantitative DB assay. Material and Methods Dot blot (DB) strips for antibodies directed to human immuno...
متن کاملquantification analysis of dot blot assays for human immunodeficiency virus type 1 and 2 antibodies
objective dot blot (db) assay provides highly specific results, but usually not reliable for quantification of antibody production. the need for a more objective db assay to provide a better definition of the immune status, against hiv antigens, promoted this study to be done to develop a quantitative db assay. material and methods dot blot (db) strips for antibodies directed to human immunodef...
متن کاملQuantification Analysis of Dot Blot Assays for Human Immunodeficiency Virus Type 1 and 2 Antibodies
Objective Dot Blot (DB) assay provides highly specific results, but usually is not reliable for quantification of antibody production. The need for a more objective DB assay to provide a better definition of the immune status, against HIV antigens, promoted this study to develop a quantitative DB assay. Materials and Methods Dot blot (DB) strips for antibodies, directed to human immunodeficienc...
متن کاملPassive hemagglutination test for detection of antibodies to human immunodeficiency virus type 1 and comparison of the test with enzyme-linked immunosorbent assay and Western blot (immunoblot) analysis.
A passive hemagglutination test (PHA) was developed for detecting antibodies to human immunodeficiency virus type 1 (HIV-1) utilizing sheep erythrocytes cross-linked with purified envelope glycoprotein (gp160) of HIV-1. In an analysis of 216 human serum samples, 100% correlation was observed in 86 reactive and 124 nonreactive serum samples between PHA and commercial enzyme-linked immunosorbent ...
متن کاملHuman immunodeficiency virus western blot tests: comparisons and considerations.
UNLABELLED Human immunodeficiency virus (HIV) Western Blot (WB) tests are used as an aid in the diagnosis of acquired immunodeficiency disease syndrome (AIDS) and related disorders. The object of this study is to examine and compare the results of four commercially available HIV WB methods. Sera from 974 persons were tested by four (Kits I, II, III, and IV) HIV WB methods from different vendors...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1987
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.25.10.1993-1998.1987